About this Flowcell

Flowcell: 42BVRAAXX

Instrument: Rotifer (ILLUMINA-33A494)
Instrument Model: Illumina Genome Analyzer II

Image Analysis: Firecrest 1.4.0
Base Caller: Bustard 1.4.0
Alignment: GERALD 1.51
Run Date: July 21, 2009, 1:30 p.m.
Type: Single
Read Length: 76
Control Lane: 7
Notes:

Multiplexing sequencing protocol

330421 4750917 SR-0004466
328461 4798321 RGT-0192923
328479 4798339 RGT-0192606
328479 4798339 RGT-0192686
328461 4798321 RGT-0192892

Control Lane 7

Abnormally high error rates in the first 10 cycles. Were able to recover sequences starting firecrest from cycle 12.  Flowcell replaced.

About this lane

Lane: 1
pM: 7.00
Cluster Estimate: 171,000
Comments: Abnormally high error rates in the first 10 cycles. Were able to recover sequences starting firecrest from cycle 12.

Library Name

Library ID: 10312
Name: Paired ends FI_AU_4_F_MB4641_s1
Affiliations:

Anna CT Abelin/Nicole Tetreault ( Allman )
Brian Williams ( Barbara Wold )

Accessions:

Sample Details

Species: Homo sapiens
Experiment Type: RNA-seq
Background or Cell Line: Unknown
Condition: Unknown
Replicate: 1

Library Details

Library Type: None
Multiplex Index:
Creation Date July 18, 2008
Made By: Brian Williams
Gel Cut Size: 400
Concentration: 22.80 ng/µl
Protocol Stopping Point Ligation, gel, then 18x PCR

Run 090721_HWI-EAS229_0125_42BVRAAXX