About this Flowcell

Flowcell: AACF2N3M5
Instrument: NextSeq2000 (Tardigrade)
Instrument Model: Illumina NextSeq2000
Run Date: Dec. 21, 2022, 11:45 a.m.
Type: Single
Read Length: 100
Control Lane: None
Notes: 
P2 100 cycles. 101-6. 500 pM. 16.66% PhiX

Custom primer 'CRISPRi_seq V5 extended' supplied by Katie at 100 uM (3/9/22). In box next to library 24223.
Nextera Read 1 Primer (Igor, IDT) at 100 uM.
Genomic DNA Sequencing Primer (Igor, IDT) at 100 uM.
Use HT1 to dilute each custom read primer mix to yield 600 μl at 0.3 μM final concentration. Use 3 ul of each primer in 1 ml of HT1. Add 550 ul of HT1/primers to custom well 1 of NextSeq cartridge

About this lane

Lane: 1
pM: 5.00

Library Name

Library ID: 19887
Name: PhiX control v3 2 nM
Affiliations:
  • Igor Antoshechkin ( GEC )
Accessions:

Sample Details

Species: Phage
Experiment Type: Whole Genome

Library Details

Library Type: None
Multiplex Index:
Creation Date Jan. 19, 2018
Made By: Illumina
Protocol Stopping Point Completed