About this Flowcell

Flowcell: AACF2N3M5
Instrument: NextSeq2000 (Tardigrade)
Instrument Model: Illumina NextSeq2000
Run Date: Dec. 21, 2022, 11:45 a.m.
Type: Single
Read Length: 100
Control Lane: None
Notes: 
P2 100 cycles. 101-6. 500 pM. 16.66% PhiX

Custom primer 'CRISPRi_seq V5 extended' supplied by Katie at 100 uM (3/9/22). In box next to library 24223.
Nextera Read 1 Primer (Igor, IDT) at 100 uM.
Genomic DNA Sequencing Primer (Igor, IDT) at 100 uM.
Use HT1 to dilute each custom read primer mix to yield 600 μl at 0.3 μM final concentration. Use 3 ul of each primer in 1 ml of HT1. Add 550 ul of HT1/primers to custom well 1 of NextSeq cartridge

About this lane

Lane: 1
pM: 5.00

Library Name

Library ID: 25241
Name: etc_TOP_CRISPRi_Alina_12192022
Affiliations:
  • Alina Guna ( Voorhees )
Accessions:

Sample Details

Species: Homo sapiens
Experiment Type: Amplicons

Library Details

Library Type: Barcoded Illumina
Multiplex Index: 14:AGTTCC
Creation Date Dec. 19, 2022
Made By: Alina Guna
Gel Cut Size: 276
Concentration: 12.10 ng/µl
Protocol Stopping Point Completed