About this Flowcell

Flowcell: AACHC2KM5
Instrument: NextSeq2000 (Tardigrade)
Instrument Model: Illumina NextSeq2000
Run Date: Jan. 24, 2023, 12:10 p.m.
Type: Single
Read Length: 100
Control Lane: None
Notes: 
P2 100 cycles. 101-6. 500 pM. 16.66% PhiX

Custom primer 'CRISPRi_seq V5 extended' supplied by Katie at 100 uM (3/9/22). In box next to library 24223.
Nextera Read 1 Primer (Igor, IDT) at 100 uM.
Genomic DNA Sequencing Primer (Igor, IDT) at 100 uM.
Use HT1 to dilute each custom read primer mix to yield 600 μl at 0.3 μM final concentration. Use 2 ul of each primer in 667 ul of HT1. Add 550 ul of HT1/primers to custom well 1 of NextSeq cartridge

About this lane

Lane: 1
pM: 5.00

Library Name

Library ID: 25294
Name: KP327_E2_BOTTOM
Affiliations:
  • Katharine Page ( Voorhees )
Accessions:

Sample Details

Species: Homo sapiens
Experiment Type: Amplicons

Library Details

Library Type: Barcoded Illumina
Multiplex Index: 6:GCCAAT
Creation Date Jan. 23, 2023
Made By: Katharine Page
Gel Cut Size: 347
Concentration: 19.50 ng/µl
Protocol Stopping Point Completed