About this Flowcell

Flowcell: 42BVRAAXX

Instrument: Rotifer (ILLUMINA-33A494)
Instrument Model: Illumina Genome Analyzer II

Image Analysis: Firecrest 1.4.0
Base Caller: Bustard 1.4.0
Alignment: GERALD 1.51
Run Date: July 21, 2009, 1:30 p.m.
Type: Single
Read Length: 76
Control Lane: 7
Notes:

Multiplexing sequencing protocol

330421 4750917 SR-0004466
328461 4798321 RGT-0192923
328479 4798339 RGT-0192606
328479 4798339 RGT-0192686
328461 4798321 RGT-0192892

Control Lane 7

Abnormally high error rates in the first 10 cycles. Were able to recover sequences starting firecrest from cycle 12.  Flowcell replaced.

About this lane

Lane: 7
pM: 7.00
Cluster Estimate: 148,000
Comments: Abnormally high error rates in the first 10 cycles. Were able to recover sequences starting firecrest from cycle 12.

Library Name

Library ID: 10954
Name: Paired End BY4741-WT a
Affiliations:

Martin Budd ( Judy Campbell )

Accessions:

Sample Details

Species: Saccharomyces cerevisiae
Experiment Type: Whole Genome
Background or Cell Line: Unknown
Condition: Unknown
Replicate: 1

Library Details

Library Type: None
Multiplex Index:
Creation Date July 2, 2009
Made By: Lorian
Gel Cut Size: 425
Concentration: 22.50 ng/µl
Protocol Stopping Point Ligation, gel, then 18x PCR

Run 090721_HWI-EAS229_0125_42BVRAAXX