About this Flowcell

Flowcell: HCJTGBCX3
Instrument: Volvox 2500 (SN787)
Instrument Model: Illumina HiSeq 2500
Run Date: Jan. 29, 2020, 1:05 p.m.
Type: Single
Read Length: 50
Control Lane: None
Notes: 
Flowcell B. 6 pM. SR50. 150 M requested.
Custom primer oCRISPRi_seq V5 extended (supplied as 50 uM). Final concentration in HP10 0.5 uM (20 ul 50 uM in 2 ml HP10).
The primer is extended 1 nt compared to the 11/19/19 run to avoid complexity problems during the first cycle.

About this lane

Lane: 1
pM: 5.00

Library Name

Library ID: 22359
Name: Top_Arm3_NMD_CRISPRi_Screen_24_01_2020
Affiliations:
  • Angel Galvez Merchan ( Voorhees )
Accessions:

Sample Details

Species: Homo sapiens
Experiment Type: Amplicons

Library Details

Library Type: Barcoded Illumina
Multiplex Index: 10:TAGCTT
Creation Date Jan. 27, 2020
Made By: Angel Galvez Merchan
Gel Cut Size: 281
Concentration: 12.60 ng/µl
Protocol Stopping Point Completed