About this Flowcell

Flowcell: HHNJGBCX3
Instrument: Volvox 2500 (SN787)
Instrument Model: Illumina HiSeq 2500
Run Date: Oct. 16, 2020, 12:29 p.m.
Type: Single
Read Length: 50
Control Lane: None
Notes: 
Flowcell B. 8 pM. SR50.
Custom primer 'CRISPRi_seq V5 extended' supplied by Katie at 100 uM. Final concentration in HP10 0.5 uM (10 ul 100 uM in 2 ml HT1).
The primer is extended 1 nt compared to the 11/19/19 run to avoid complexity problems during the first cycle. It is the same as the one used for Angels's run on 1/29/20, but different dilution.

About this lane

Lane: 1
pM: 5.00

Library Name

Library ID: 22652
Name: Top_Arm1__KP35_Screen_CRISPRI_Katie_09.28.20
Affiliations:
  • Katharine Page ( Voorhees )
Accessions:

Sample Details

Species: Homo sapiens
Experiment Type: Amplicons

Library Details

Library Type: Barcoded Illumina
Multiplex Index: 12:CTTGTA
Creation Date Sept. 30, 2020
Made By: Katharine Page
Gel Cut Size: 276
Concentration: 21.80 ng/µl
Protocol Stopping Point Completed