About this Flowcell

Flowcell: HKH35BCX3
Instrument: Volvox 2500 (SN787)
Instrument Model: Illumina HiSeq 2500
Run Date: Aug. 12, 2021, 12:15 p.m.
Type: Single
Read Length: 50
Control Lane: None
Notes: 
Flowcell B. 7.5 pM. SR50.
Custom primer 'CRISPRi_seq V5 extended' supplied by Katie at 100 uM (8/11/21). Final concentration in HP10 0.5 uM (10 ul 100 uM in 2 ml HP10 - using HP10 instead of HT1 because of the addition of PhiX this time).
The primer is extended 1 nt compared to the 11/19/19 run to avoid complexity problems during the first cycle. It is the same as the one used for Angels's run on 1/29/20, but different dilution.

About this lane

Lane: 1
pM: 5.00

Library Name

Library ID: 23529
Name: Index #10 Bottom_A1_CRISPRi_KP28
Affiliations:
  • Katharine Page ( Voorhees )
Accessions:

Sample Details

Species: Homo sapiens
Experiment Type: Amplicons

Library Details

Library Type: NEBNext Multiplexed
Multiplex Index: 10:TAGCTT
Creation Date Aug. 11, 2021
Made By: Katharine Page
Gel Cut Size: 276
Concentration: 13.10 ng/µl
Protocol Stopping Point Completed