About this Flowcell

Flowcell: HN3HVBCX3
Instrument: Volvox 2500 (SN787)
Instrument Model: Illumina HiSeq 2500
Run Date: Oct. 14, 2021, 1:41 p.m.
Type: Single
Read Length: 50
Control Lane: None
Notes: 
Flowcell A. 7.5 pM. SR50.
Custom primer 'YC09_CRISPRi_seq_v5 extended' supplied by Yoga at 100 uM (10/05/21). Final concentration in HP10 0.5 uM (10 ul 100 uM in 2 ml HP10 - using HP10 instead of HT1 because of the addition of PhiX this time).
The primer is extended 1 nt compared to the 11/19/19 run to avoid complexity problems during the first cycle. It is the same as the one used for Angels's run on 1/29/20, but different dilution. And for Katie's runs.

About this lane

Lane: 1
pM: 5.00

Library Name

Library ID: 23611
Name: Index #12 Top_DFP_Arm2_Screen_CRISPRi_YC_10052021
Affiliations:
  • Yogaditya Chakrabarty ( Chan )
Accessions:

Sample Details

Species: Homo sapiens
Experiment Type: Amplicons

Library Details

Library Type: Barcoded Illumina
Multiplex Index: 12:CTTGTA
Creation Date Oct. 5, 2021
Made By: Yogaditya Chakrabarty
Gel Cut Size: 274
Concentration: 26.60 ng/µl
Protocol Stopping Point Completed