About this Flowcell

Flowcell: H97W7ADXX
Instrument: Volvox 2500 (SN787)
Instrument Model: Illumina HiSeq 2500
Run Date: April 22, 2014, 3:37 p.m.
Type: Single
Read Length: 100
Control Lane: None
Notes: 
Flowcell B. On-board clustering. 11 pM.
Single library containing two samples of bisulfite-converted genomic DNA from mouse male germ cells made with NuGen Methyl-Seq kit. Barcodes BC2 and BC6 (NuGen) - not ideal combination.
Read 1 requires custom MetSeq Primer 1 supplied at 25 uM. Use at final concentration of 0.5 uM (50X). Add 35 ul of primer to 1.75 ml HP10 before sequencing.

About this lane

Lane: 1
pM: 11.00
Comments: Custom MethSeq primer added to HP10 to final concentration of 0.5 uM.

Library Name

Library ID: 14426
Name: bis-seq_miwi2_germ10d_Het-KO
Affiliations:
  • Dubravka Pezic ( Aravin )
Accessions:

Sample Details

Species: Mus musculus
Experiment Type: Unknown

Library Details

Library Type: NuGen DR
Multiplex Index: BC2:TGGTGA BC6:GTGCTT
Creation Date April 21, 2014
Made By: Dubravka Pezic
Gel Cut Size: 280
Concentration: 17.40 ng/µl
Protocol Stopping Point Completed