About this Flowcell
Flowcell: AACHC2KM5
Instrument:
NextSeq2000
(Tardigrade)
Instrument Model: Illumina NextSeq2000
Instrument Model: Illumina NextSeq2000
Type: Single
Read Length: 100
Control Lane: None
Notes:
P2 100 cycles. 101-6. 500 pM. 16.66% PhiX Custom primer 'CRISPRi_seq V5 extended' supplied by Katie at 100 uM (3/9/22). In box next to library 24223. Nextera Read 1 Primer (Igor, IDT) at 100 uM. Genomic DNA Sequencing Primer (Igor, IDT) at 100 uM. Use HT1 to dilute each custom read primer mix to yield 600 μl at 0.3 μM final concentration. Use 2 ul of each primer in 667 ul of HT1. Add 550 ul of HT1/primers to custom well 1 of NextSeq cartridge
Lanes
| Lane | Library ID | Library Name | Species | Comment | Lane Status |
| 1 | 25296 | KP327_NT_BOTTOM | Homo sapiens | ||
| 1 | 25295 | KP327_NT_TOP | Homo sapiens | ||
| 1 | 25294 | KP327_E2_BOTTOM | Homo sapiens | ||
| 1 | 25293 | KP327_E2_TOP | Homo sapiens | ||
| 1 | 19887 | PhiX control v3 2 nM | Phage |