About this Flowcell

Flowcell: AAH2CNTM5
Instrument: NextSeq2000 (Tardigrade)
Instrument Model: Illumina NextSeq2000
Run Date: May 29, 2025, 12:44 p.m.
Type: Paired
Read Length: 300
Control Lane: None
Notes: 
P1 600 cycles. 301-12-301 550 pM. 15% PhiX. XLEAP-SBS
Add 550 ul of R1/R2 primers to custom well 1 of NextSeq cartridge
Add 550 ul of I1 primer to custom well 2 of NextSeq cartridge

Nextera Read 1 Primer (Igor, IDT) at 100 uM: TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG
Genomic DNA Sequencing Primer (Igor, IDT) at 100 uM: ACACTCTTTCCCTACACGACGCTCTTCCGATCT

Nextera Read2 Primer (IDT, not HPLC-purified, provided by Matt but designed by Igor) at 100 uM: GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG
Genomic DNA Read2 Sequencing Primer (IDT, not HPLC-purified, provided by Matt but designed by Igor) at 100 uM: GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT

16S Read 1 primer priovided by Matt at 100 uM
16S Read 2 primer priovided by Matt at 100 uM

16S Indices primer priovided by Matt at 100 uM

Lanes

Lane Library ID Library Name Species Comment Lane Status
1 27627 20250518_16S_DARPA_Final_Pool Unknown
1 19887 PhiX control v3 2 nM Phage