HTSWorkflow

About this Flowcell

Flowcell: HJKJLBCX3
Run Date: Dec. 14, 2020, 12:46 p.m.
Type: Single
Read Length: 50
Control Lane: None
Notes:

Flowcell A. 7.5 pM. SR50.
Custom primer 'CRISPRi_seq V5 extended' supplied by Katie at 100 uM. Final concentration in HP10 0.5 uM (10 ul 100 uM in 2 ml HP10 - using HP10 instead of HT1 because of the addition of PhiX this time).
The primer is extended 1 nt compared to the 11/19/19 run to avoid complexity problems during the first cycle. It is the same as the one used for Angels's run on 1/29/20, but different dilution.

Flowcell A.