About this Flowcell

Flowcell: AACF2N3M5
Instrument: NextSeq2000 (Tardigrade)
Instrument Model: Illumina NextSeq2000
Run Date: Dec. 21, 2022, 11:45 a.m.
Type: Single
Read Length: 100
Control Lane: None
Notes: 
P2 100 cycles. 101-6. 500 pM. 16.66% PhiX

Custom primer 'CRISPRi_seq V5 extended' supplied by Katie at 100 uM (3/9/22). In box next to library 24223.
Nextera Read 1 Primer (Igor, IDT) at 100 uM.
Genomic DNA Sequencing Primer (Igor, IDT) at 100 uM.
Use HT1 to dilute each custom read primer mix to yield 600 μl at 0.3 μM final concentration. Use 3 ul of each primer in 1 ml of HT1. Add 550 ul of HT1/primers to custom well 1 of NextSeq cartridge

Lanes

Lane Library ID Library Name Species Comment Lane Status
1 25242 etc_BOTTOM__CRISPRi_Alina_12192022 Homo sapiens
1 25241 etc_TOP_CRISPRi_Alina_12192022 Homo sapiens
1 25240 mtx_BOTTOM_CRISPRi_Alina_12192022 Homo sapiens
1 25239 mtx_TOP_CRISPRi_Alina_12192022 Homo sapiens
1 19887 PhiX control v3 2 nM Phage